GenoType^® MTBDRsl - Your Important Assistance for Detetion of XDR-TB

Identification of the M. tuberculosis complex and its resistance to fluoroquinolones, aminoglycosides/cyclic peptides and ethambutol

The emergence and spread of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) are a major medical and public problem threatening the global health.
MDR-TB is defined as TB that is resistant to the two first-line anti-TB drugs rifampicin and isoniazid. XDR-TB strains are in addition resistant to fluoroquinolones and injectable second-line drugs. XDR-TB emerges through mismanagement of MDR-TB treatment.
Conventional methods for mycobacteria culture and drug susceptibility testing are slow and elaborate, requiring sequential procedures for the diagnosis. During this time patients may be treated inappropriately, drug resistant strains may continue to spread, and amplification of resistance may occur. Therefore rapid diagnosis and identification of MDR-TB or XDR-TB strains are prerequisites for the worldwide fight against TB.

The GenoType^® MTBDRsl gives you the possibility to diagnose patients with MDR-TB to receive information on further antibiotic resistances, to test patient after therapy failure and relapse as well as to diagnose patients from countries with high usage of the respective drugs. 

The identification of resistance to fluoroquinolones is enabled by the detection of the most significant mutations of the gyrA gene (coding for DNA gyrase). For the detection of resistance to aminoglycosides/cyclic peptides, the 16S rRNA gene (rrs) and for detection of resistance to ethambutol the embB gene (which, together with the genes embA and embC, codes for arabinosyl transferase) are examined.

Your benefits with GenoType^® MTBDRsl

• Detection of XDR-TB in patients previously diagnosed with an MDR-TB by testing with the GenoType^® MTBDRplus.
• Confirmation of DST results.

At a glance
Molecular genetic assay for identification of resistance to fluoroquinolones, aminoglycosides/cyclic peptides and ethambutol of Mycobacterium tuberculosis complex

Starting material:
decontaminated pulmonary smear-positive specimens and/or liquid/solid culture samples

DNA Isolation:
quick protocol (for further informaton please refer to the manual)

Order number:
12 tests  No. 317
96 tests  No. 31796