Your test system for the detection of Shiga toxin genes stx1 and stx2, the intimin gene eae as well as the ipaH gene
Approximately 1.000 cases of infection caused by enterohaemorrhagic E. coli (EHEC) occur every year in Germany.
EHEC are highly contagious. Generally, 10 to 100 pathogens are enough to trigger an infection and possibly serious complications, such as haemolytic-uraemic syndrome (HUS). Improved laboratory diagnostics are therefore indispensable for the early diagnosis of the disease, in order to allay the clinical course and to avoid outbreaks, especially in public facilities.
Microbiological detection of EHEC is difficult, since EHEC can not be phenotypically distinguished from apathogenic E. coli. The most important diagnostic criteria in EHEC laboratory diagnostics has to be the detection of shiga toxins. For the identification of shiga toxins the use of molecular methods is recommended.
With our test system GenoType EHEC, such a method is available to you for your reliable and rapid EHEC diagnostics. Starting from cultivated samples, the Shiga toxin genes, that are important for the reliable identification of an EHEC infection, are detected. The differentiation of EHEC from Shigella ssp. and EIEC is ensured with the detection of the Shigella-specific virulence factor ipaH.
Your benefits with GenoType EHEC
- Unambiguous results: A reliable differentiation of E. coli and Shigella ssp. as well as the detection of the diagnostically significant Shiga toxin genes and the intimin gene eae provides you with unambiguous results. Thus, an efficient EHEC diagnostics is ensured.
- Rapid result: Detection is performed using culture material within one working day and thus allows for the rapid detection of EHEC.
- Cost-efficient: No cost-intensive instrumentation is requird for the procedure; consequently, economic set-up is also possible for smaller laboratories.
At a glance
Molecular genetic assay for identification of Shiga toxin genes stx1 and stx2, the eae gene and the ipaH gene
culture, preferred bacteria freshly grown
quick protocol (for further informaton please refer to the manual)
12 tests No. 344
96 tests No. 34496